In commemoration of the World Rabies Day, the WHO CC and the WOAH Reference Laboratory for Rabies at the Friedrich-Loeffler-Institut, Germany, together with the Botswana Veterinary Research Institute, Botswana, organised a workshop on rabies diagnosis, which took place in Gaborone from 20 to 22 September 2022. Participants from Zambia, Namibia, Angola and Botswana were trained on rabies surveillance, sampling methods, DFA and real-time PCR. The workshop was considered a great success and the participants expressed the wish to expand and intensify this type of cooperation in the coming years.